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anti pstat3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pstat3
    Anti Pstat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pstat3/pm41936612-101-12-14
    Average 86 stars, based on 1 article reviews
    anti pstat3 - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Histone methyltransferase EZH2 drives podocyte injury and senescence in diabetic nephropathy through STAT3 activation.
    Article Snippet: .. The following antibodies were used for immunofluorescence staining: EZH2 (1:100, 5246S, CST, USA), pSTAT3 (1:50, 4113S, CST, USA), podocin (1:200, ab50339, Abcam, UK), WT-1 (1:200, ab89901, Abcam, UK), Flag (1:200, 80010-1-RR, Proteintech, Wuhan, China) and B7-1 (1:200, sc-376012, Santa Cruz Biotechnology, USA). .. Finally, images were captured and analyzed using a Zeiss LSM880 confocal microscope (Carl Zeiss, Germany).

    Article Title: Histone methyltransferase EZH2 drives podocyte injury and senescence in diabetic nephropathy through STAT3 activation
    Article Snippet: .. The following antibodies were used for immunofluorescence staining: EZH2 (1:100, 5246 S, CST, USA), pSTAT3 (1:50, 4113 S, CST, USA), podocin (1:200, ab50339, Abcam, UK), WT-1 (1:200, ab89901, Abcam, UK), Flag (1:200, 80010-1-RR, Proteintech, Wuhan, China) and B7-1 (1:200, sc-376012, Santa Cruz Biotechnology, USA). .. Finally, images were captured and analyzed using a Zeiss LSM880 confocal microscope (Carl Zeiss, Germany).

    Staining:

    Article Title: Histone methyltransferase EZH2 drives podocyte injury and senescence in diabetic nephropathy through STAT3 activation.
    Article Snippet: .. The following antibodies were used for immunofluorescence staining: EZH2 (1:100, 5246S, CST, USA), pSTAT3 (1:50, 4113S, CST, USA), podocin (1:200, ab50339, Abcam, UK), WT-1 (1:200, ab89901, Abcam, UK), Flag (1:200, 80010-1-RR, Proteintech, Wuhan, China) and B7-1 (1:200, sc-376012, Santa Cruz Biotechnology, USA). .. Finally, images were captured and analyzed using a Zeiss LSM880 confocal microscope (Carl Zeiss, Germany).

    Article Title: Histone methyltransferase EZH2 drives podocyte injury and senescence in diabetic nephropathy through STAT3 activation
    Article Snippet: .. The following antibodies were used for immunofluorescence staining: EZH2 (1:100, 5246 S, CST, USA), pSTAT3 (1:50, 4113 S, CST, USA), podocin (1:200, ab50339, Abcam, UK), WT-1 (1:200, ab89901, Abcam, UK), Flag (1:200, 80010-1-RR, Proteintech, Wuhan, China) and B7-1 (1:200, sc-376012, Santa Cruz Biotechnology, USA). .. Finally, images were captured and analyzed using a Zeiss LSM880 confocal microscope (Carl Zeiss, Germany).

    Western Blot:

    Article Title: Antibody-based compositions for targeting tropomyosin receptor kinase B (TrkB) isoforms
    Article Snippet: Antibodies For TrkB.T1 IHC, the generated mouse TrkB.T1 antibody was used (1.3 mg/ml) at 1:20 and recombinant rabbit fusion SPEH1 D12 (11.5 mg/ml) was used at 1:500 and TrkB kinase antibody (abcam #ab18987; lot: GR3280550-2) at 1:250. .. For western blot analyses of human brain, mouse brain, and 3T3-Tv-a cells, commercial antibodies were used according to manufacturer specifications and bands were confirmed by size using SpectraTM Multicolor Broad Range Protein Ladder (ThermoScientificTM catalog #26634; lot: 00784968): pSTAT3 (Tyr705, Cell Signaling #9145; lot: 22 at 1:1000), pAkt (Ser473, Cell Signaling #4060; lot: 23 at 1:1000), pERK (Cell Signaling phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E) XP® #4370; lot: 12 at 1:2000), β-actin (Sigma #AB1978 Clone AC-15; lot: 021M4821 at 1:10,000), pS6 ribosomal protein (Ser235/236, Cell Signaling #4858; lot: 16 at 1:1000), TrkB (Millipore #07-225; lot: 2187222 and lot: 3277578 @ 1:3000), TrkB (abcam #ab33655; lot: GR266297-1 at 1:1000), TrkB (Abcam #ab18987; lot: GR3280550-2 at 1:2000), pPDGFR (3 (Y1021; Abcam #ab62437; lot: GR38791-13 at 1:200), PDGFR 13 (Cell Signaling #3169; lot: 13 at 1:800), ERBB3 (Cell Signaling #12708; lot: 4 at 1:1000), NRG2 (Abcam #ab220615; lot: GR3181158-4 at 1:200), vinculin (Sigma-Aldrich, Cat. V9131; lot: 118M4777V at 1:10.000) and added in 5% BSA/TBST overnight at 4° C. Primary Nestin/Tv-a Neurospheres Neural progenitors were isolated from Nestin(N)/tv-apups at P0. ..

    Isolation:

    Article Title: Antibody-based compositions for targeting tropomyosin receptor kinase B (TrkB) isoforms
    Article Snippet: Antibodies For TrkB.T1 IHC, the generated mouse TrkB.T1 antibody was used (1.3 mg/ml) at 1:20 and recombinant rabbit fusion SPEH1 D12 (11.5 mg/ml) was used at 1:500 and TrkB kinase antibody (abcam #ab18987; lot: GR3280550-2) at 1:250. .. For western blot analyses of human brain, mouse brain, and 3T3-Tv-a cells, commercial antibodies were used according to manufacturer specifications and bands were confirmed by size using SpectraTM Multicolor Broad Range Protein Ladder (ThermoScientificTM catalog #26634; lot: 00784968): pSTAT3 (Tyr705, Cell Signaling #9145; lot: 22 at 1:1000), pAkt (Ser473, Cell Signaling #4060; lot: 23 at 1:1000), pERK (Cell Signaling phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E) XP® #4370; lot: 12 at 1:2000), β-actin (Sigma #AB1978 Clone AC-15; lot: 021M4821 at 1:10,000), pS6 ribosomal protein (Ser235/236, Cell Signaling #4858; lot: 16 at 1:1000), TrkB (Millipore #07-225; lot: 2187222 and lot: 3277578 @ 1:3000), TrkB (abcam #ab33655; lot: GR266297-1 at 1:1000), TrkB (Abcam #ab18987; lot: GR3280550-2 at 1:2000), pPDGFR (3 (Y1021; Abcam #ab62437; lot: GR38791-13 at 1:200), PDGFR 13 (Cell Signaling #3169; lot: 13 at 1:800), ERBB3 (Cell Signaling #12708; lot: 4 at 1:1000), NRG2 (Abcam #ab220615; lot: GR3181158-4 at 1:200), vinculin (Sigma-Aldrich, Cat. V9131; lot: 118M4777V at 1:10.000) and added in 5% BSA/TBST overnight at 4° C. Primary Nestin/Tv-a Neurospheres Neural progenitors were isolated from Nestin(N)/tv-apups at P0. ..

    Incubation:

    Article Title: Role of STAT1 in modulating the host immune response to Plasmodium yoelii 17XL-infected murine blood-stage malaria.
    Article Snippet: .. Primary antibodies against STAT1, p-STAT1, STAT3, and pSTAT3 (Cell Signaling Technology, USA) were incubated with the membrane at a 1:1,000 dilution overnight at 4°C. .. A horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (Southern Biotech, USA) was used for detection at a 1:5,000 dilution for 1 h at room temperature (RT).

    Membrane:

    Article Title: Role of STAT1 in modulating the host immune response to Plasmodium yoelii 17XL-infected murine blood-stage malaria.
    Article Snippet: .. Primary antibodies against STAT1, p-STAT1, STAT3, and pSTAT3 (Cell Signaling Technology, USA) were incubated with the membrane at a 1:1,000 dilution overnight at 4°C. .. A horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (Southern Biotech, USA) was used for detection at a 1:5,000 dilution for 1 h at room temperature (RT).



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    Image Search Results


    CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Journal: The FASEB Journal

    Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

    doi: 10.1096/fj.202600333R

    Figure Lengend Snippet: CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Article Snippet: After washing, cells were incubated overnight at 4°C with primary antibodies against CSPG4 (Abcam, ab275024, 1:400), nectin‐3 (PVRL3, Invitrogen, PA5‐51095, 1:200), LRP1 (Abcam, ab92544, 1:100), NFκB p65 (Santa Cruz Biotechnology, sc‐372, 1:100), or pSTAT3 (R&D Systems, AF4607, 1:100).

    Techniques: Translocation Assay, Immunostaining, Staining, Incubation, Control